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well primary antibodies  (R&D Systems)


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    R&D Systems well primary antibodies
    Well Primary Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/well primary antibodies/product/R&D Systems
    Average 93 stars, based on 45 article reviews
    well primary antibodies - by Bioz Stars, 2026-03
    93/100 stars

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    Representative semiquantitative immunoblotting of kidney: ( a ) BSC-1, ( b ) Type 3 Na + /H + exchanger (NHE3), ( c ) TSC, ( d ) Na + -K + -ATPase, ( e ) <t>α-ENaC,</t> ( f ) β-ENaC, and ( g ) <t>γ-ENaC.</t> DU, densitometric unit; P, positive control; BSC-1, bumetanide-sensitive cotransporter type 1; TSC, thiazide-sensitive Na-Cl cotransporter; ENaC, epithelial sodium channel. * p < 0.05, compared to the control group. @ p < 0.05, compared to the SD group. # p < 0.05, compared to the EG group.
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    Representative semiquantitative immunoblotting of kidney: ( a ) BSC-1, ( b ) Type 3 Na + /H + exchanger (NHE3), ( c ) TSC, ( d ) Na + -K + -ATPase, ( e ) <t>α-ENaC,</t> ( f ) β-ENaC, and ( g ) <t>γ-ENaC.</t> DU, densitometric unit; P, positive control; BSC-1, bumetanide-sensitive cotransporter type 1; TSC, thiazide-sensitive Na-Cl cotransporter; ENaC, epithelial sodium channel. * p < 0.05, compared to the control group. @ p < 0.05, compared to the SD group. # p < 0.05, compared to the EG group.
    Buffer Of The Primary Antibody And Secondary Antibody As Well As Blocking Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    primary antibodies to gtp cyclohydrolase-1 (gtpch1; 1:250, santa cruz biotechnology, santa cruz, ca, 100 µg protein/well)  (Santa Cruz Biotechnology)
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    Primary Antibodies To Gtp Cyclohydrolase 1 (Gtpch1; 1:250, Santa Cruz Biotechnology, Santa Cruz, Ca, 100 µg Protein/Well), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies to gtp cyclohydrolase-1 (gtpch1; 1:250, santa cruz biotechnology, santa cruz, ca, 100 µg protein/well)/product/Santa Cruz Biotechnology
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    Image Search Results


    Representative semiquantitative immunoblotting of kidney: ( a ) BSC-1, ( b ) Type 3 Na + /H + exchanger (NHE3), ( c ) TSC, ( d ) Na + -K + -ATPase, ( e ) α-ENaC, ( f ) β-ENaC, and ( g ) γ-ENaC. DU, densitometric unit; P, positive control; BSC-1, bumetanide-sensitive cotransporter type 1; TSC, thiazide-sensitive Na-Cl cotransporter; ENaC, epithelial sodium channel. * p < 0.05, compared to the control group. @ p < 0.05, compared to the SD group. # p < 0.05, compared to the EG group.

    Journal: International Journal of Molecular Sciences

    Article Title: Long-Term Sodium Deficiency Reduces Sodium Excretion but Impairs Renal Function and Increases Stone Formation in Hyperoxaluric Calcium Oxalate Rats

    doi: 10.3390/ijms25073942

    Figure Lengend Snippet: Representative semiquantitative immunoblotting of kidney: ( a ) BSC-1, ( b ) Type 3 Na + /H + exchanger (NHE3), ( c ) TSC, ( d ) Na + -K + -ATPase, ( e ) α-ENaC, ( f ) β-ENaC, and ( g ) γ-ENaC. DU, densitometric unit; P, positive control; BSC-1, bumetanide-sensitive cotransporter type 1; TSC, thiazide-sensitive Na-Cl cotransporter; ENaC, epithelial sodium channel. * p < 0.05, compared to the control group. @ p < 0.05, compared to the SD group. # p < 0.05, compared to the EG group.

    Article Snippet: Primary antibodies for NHE3, TSC, and BSC-1, as well as α, β, and γ-ENaC, were purchased from Alpha Diagnostic International (San Antonio, TX, USA); beta-actin was purchased from Sigma Chemical Co. (St. Louis, MO, USA), and AQP was purchased from Alomone Labs Ltd. (Jerusalem, Israel).

    Techniques: Western Blot, Positive Control, Control

    Response of cytotoxic T lymphocytes stimulated by neoantigen peptide-pulsed mature dendritic cells (mDCs). ( a ) IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by neoantigen peptide-pulsed mDCs. The final concentration of peptide was 50 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( b ) Dose-dependent increase in the IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by peptide 3- or 9-pulsed mDCs. The final concentrations of peptides were 5, 50, and 100 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( c ) Cytotoxicity assay of CTLs induced by 4 weeks of coculture with neoantigen-pulsed mDCs. Numbers of calcein-positive living tumour cells were significantly decreased by co-culture with CTLs stimulated with peptide 9-pulsed DCs. The mean % of cytotoxicity ± SEM is shown (n = 3–5). ( d ) IFNγ-release responses by peptide 9-pulsed mDC stimulated with CTLs against autologous tumour cells. Ly: lymphocyte; mDC, mature dendritic cell; TC, tumour cell; CTL/P9, CTLs induced by peptide 9-pulsed DCs; CTL/WT, CTLs induced by wildtype peptide-pulsed DCs. The mean number of IFN-γ ELISpot ± SEM. is shown (n = 2–3).

    Journal: Scientific Reports

    Article Title: Neoantigens elicit T cell responses in breast cancer

    doi: 10.1038/s41598-021-91358-1

    Figure Lengend Snippet: Response of cytotoxic T lymphocytes stimulated by neoantigen peptide-pulsed mature dendritic cells (mDCs). ( a ) IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by neoantigen peptide-pulsed mDCs. The final concentration of peptide was 50 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( b ) Dose-dependent increase in the IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by peptide 3- or 9-pulsed mDCs. The final concentrations of peptides were 5, 50, and 100 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( c ) Cytotoxicity assay of CTLs induced by 4 weeks of coculture with neoantigen-pulsed mDCs. Numbers of calcein-positive living tumour cells were significantly decreased by co-culture with CTLs stimulated with peptide 9-pulsed DCs. The mean % of cytotoxicity ± SEM is shown (n = 3–5). ( d ) IFNγ-release responses by peptide 9-pulsed mDC stimulated with CTLs against autologous tumour cells. Ly: lymphocyte; mDC, mature dendritic cell; TC, tumour cell; CTL/P9, CTLs induced by peptide 9-pulsed DCs; CTL/WT, CTLs induced by wildtype peptide-pulsed DCs. The mean number of IFN-γ ELISpot ± SEM. is shown (n = 2–3).

    Article Snippet: Briefly, 96-well plates with nitrocellulose membranes (Millipore, Molshelm, France) precoated with a primary anti-IFN-γ antibody were pretreated with RPMI-1640 medium that containrf 10% autologous serum at 4 °C overnight.

    Techniques: Enzyme-linked Immunospot, Concentration Assay, Cytotoxicity Assay, Co-Culture Assay